I need to explain procedure from the data I submitted andThe staining procedure for the Hematoxylin and Eosin involved four stages. These stages are removal of paraffin, rehydration, staining and dehydration. Removal of paraffin involves mixing Xylene or Neo-Clear for 3 minutes. It also involves another Xylene or Neo-Clear for 2 minutes. The second stage is the rehydration which involves adding 100% ErOH, 100% ErOH for 1 minute, and 95% ErOH for 1 minute and water for 3 minutes. The staining stage involves mixing Hematoxylin for 2 minutes, and water wash for 2-5 minutes in running water. This stage also involves adding scott solution to blue hematoxylin for 2 minutes. Eosin is also added 1-3 minutes, and this is checked after 1 minute.The last is the dehydration which involves adding 1 or 2 dips of 70% ErOH, 1 or 2 dips of 70% ErOH, 1 or 2 dips of 95% ErOH and 1 or 2 dips of 95% ErOH. The rinses are performed quickly to preserve the intensity of the cosin counterstain. After rinsing, 100% ErOH is mixed for 3 minutes twice. In addition, Xylene or Neo-Clear is added for 3 minutes twice. After the four stages are done, the specimen is mounted with Cytoseal XYL and covered with a coverslip before the sample dries. explain difference between reported method and actual method followed.
